DNA-RNA Amplification Direct Amplification
Direct PCR [3G]
![Direct_PCR_[3G]](/upload_files/editor/202411/DPR200.jpg "DPR200")
No DNA extraction required. Taq pol. based product that can be applied to UDG system.
- Amplify directly from whole blood, animal and plants tissues
- Skip DNA purification
- Various genotyping
- Prevention of carryover contamination
Products
| Cat.No. | Product | Size |
|---|---|---|
| DPR200 | HelixAmp™ Direct PCR [3G] | 200 rxns |
| DPRU200 | HelixAmp™ Direct PCR [3G] [UDG System] | 200 rxns |
| DPRH200 | HelixAmp™ Direct PCR [3G] [Hot-start] | 200 rxns |
| DPRHU200 | HelixAmp™ Direct PCR [3G] [Hot-start] [UDG System] | 200 rxns |
HelixAmp™ Direct PCR [3G] is designed for the PCR amplification directly from animal tissues, whole blood, and plant tissues without any DNA purification processes. This kit contains a 2x reaction mix including Taq DNA polymerase, dNTPs, MgCl₂, and unique buffer system to resist various PCR inhibitors of tissue samples. Unlike other brands of direct PCR which based on a derivative of Pfu DNA polymerase, NanoHelix’s Direct PCR [3G] is based on Taq polymerase. Due to this enzyme’s robust amplification and 3’ to 5’ exo-negative properties, this kit could be used for allele specific PCR which is routinely used for various genotyping. Moreover the Uracil-DNA glycosylase with dUTP system for the prevention of carryover contamination can be applied to this Taq polymerase-based kit. dUTP could not be used with the Pfu DNA polymerase and its derivatives. A Uracil-DNA glycosylase and dUTP applied version is also available.
HelixAmp™ Direct PCR [3G] [Hot-start] is designed for the PCR amplification directly from animal tissues, whole blood, and plant tissues without any DNA purification processes. This kit contains a 2x reaction mix including Hot-start Taq DNA polymerase, dNTPs, MgCl₂, and unique buffer system to resist various PCR inhibitors of tissue samples. Hot-start Taq DNA polymerase is inactive at lower temperature by an inhibitory antibody and using this enzyme avoids the polymerization from non-specifically bound primers during the setting of PCR mix and at the start of PCR cycles. At high temperature of the initial denaturation step of PCR, the inhibitory antibody is released by denaturation and the free Taq DNA polymerase becomes active. NanoHelix’s Direct PCR [3G] is based on Taq polymerase. Due to this enzyme’s robust amplification and 3’ to 5’ exo-negative properties, this kit could be used for allele specific PCR which is routinely used for various genotyping. Moreover the Uracil-DNA glycosylase with dUTP system for the prevention of carryover contamination can be applied to this Taq polymerase-based kit. dUTP could not be used with the Pfu DNA polymerase and its derivatives. A Uracil-DNA glycosylase and dUTP applied version is also available.
